A week before the 2015 Boston Marathon, Jonathan Scheiman, then a postdoctoral researcher in the lab of geneticist George Church at Harvard Medical School in Boston, Massachusetts, was racing around the city in a hire car collecting faeces. He gathered stool samples from runners planning to participate, as well as those from non-runners — mostly fellow postdocs. After the race, he revisited his donors to collect more samples. Four years later, his efforts were rewarded with a paper describing one of the first attempts to establish a causal link between the symbiotic community of microorganisms living in our guts and athletic performance.
Although the gut microbiome has been implicated in numerous aspects of health and disease, links with athleticism are much less studied. Interest is growing, however, helped by advances over the past decade that enable researchers to reveal not just which microbes the gut harbours but also what they do. Such work suggests that the enormous diversity of organisms that make up a person’s gut microbiome — each as unique as a fingerprint — might converge on a smaller number of functions, which, in turn, could suggest candidate mechanisms. And although elite athletes and their coaches might hope to be the first to benefit, a deeper understanding of the link between the gut microbiome and physical fitness might instead benefit the health of the wider population.
Of the trillions of microorganisms that live on and inside us, most are found in the gastrointestinal tract. Some are pathogens, but many are beneficial, and the overall community is essential to human health. Disruption of the microbiome is directly linked to gastrointestinal conditions, such as inflammatory bowel disease, and has been implicated in diabetes, cancer, heart disease, obesity and even mental health disorders. The clearest indication of the microbes’ importance comes from laboratory mice conditioned to carry none at all. “They’ve got metabolic issues, immune issues, neurological issues — you name it,” says Aleksandar Kostic, a microbiologist at Harvard and a senior author on Scheiman’s study. “Introduce a normal mouse microbiome to them, and many of these phenotypes are rescued,” he adds.
Work to unpick the mind-boggling complexity of the gut microbiome has been greatly aided by technical advances over the past two decades, and more affordable genetic sequencing. To investigate a community, researchers must sequence the genetic code of hundreds of organisms simultaneously, drawn from a cast of millions. To identify the microbes present in a sample — usually faecal matter in the case of the gut microbiome — researchers could simply grind it up and sequence everything. Historically, this had two big drawbacks: it was very expensive, and it was difficult for researchers to work out which genes belonged to which microbes. It has been likened to emptying many jigsaws into a box, throwing away the lids, and then trying to solve the puzzles.
To avoid this problem, researchers use a technique known as marker-gene sequencing. This involves sequencing a single gene common to every microbe — specifically, the gene the codes for the 16S rRNA of the ribosome, part of the cellular machinery that translates RNA into proteins. Some parts of the gene are identical no matter which microbe it is from, allowing researchers to easily locate it in a sample. But other regions vary from one microbe to another — differences that make it possible to distinguish which organisms are present. The technique enables researchers to rapidly characterize microbiome composition at low cost. However, because it only uses one piece of each organism, the information it provides is limited. The variations in the 16S gene typically only permit classification of the microbes to the level of genus, not species.
Now, however, many researchers are fronting up to the challenge of sequencing everything. The plummeting cost of sequencing over the past decade or so, as well as the development of databases of microbial genomes, which serve as the puzzle-box lids that enable researchers to match genes to microbes, have made this approach much more tractable. Known as shotgun sequencing, this technique provides more detailed insights than 16S rRNA gene sequencing — microbes can be distinguished beyond even their species, right down to the strain, and researchers can more easily characterize their potential functions on the basis of known genes. As sequencing costs continue to fall, the technique is coming within reach of more researchers. “Within the next few years the price is going to be more competitive with 16S,” says nutrition scientist Alex Mohr at Arizona State University in Tempe.
The make-up of the gut microbiome is influenced by many factors, including how the person was born (vaginal or caesarean delivery), the use of drugs (especially antibiotics), smoking habits, alcohol consumption, stress levels, age and — most obviously — diet. Remarkably little, however, is known about how exercise affects the microbiome, or vice versa. Although the field is in its infancy, researchers are beginning to glimpse a relationship, along with potential mechanisms.
A frequent finding is that fitness is associated with greater microbial diversity. One 2016 study that used 16S rRNA gene sequencing found that, in 39 healthy adults, cardiorespiratory fitness correlated with microbial diversity. This was the case even after factors such as diet were accounted for. Variation in cardiorespiratory fitness was better than sex, age, body mass index (BMI) and diet at predicting the degree of species diversity in participants’ guts.
The team did not find specific genera or families of microbes that were significantly associated with fitness. But they were able to use a microbial database to infer the likely functions of the microbes associated with cardiorespiratory fitness, these included microbial mobility and fatty-acid synthesis. One role of gut bacteria is to help break down complex carbohydrates by fermentation, producing short-chain fatty acids (SCFAs) such as butyrate, acetate and propionate as by-products. SCFAs are emerging as a potentially important component of the link between the microbiome and fitness. Of the most common SCFAs, “propionate and butyrate can only be produced by gut bacteria,” says Kostic. These are thought to act as energy sources in muscle, he adds. The team found that fitter participants had higher levels of butyrate in their faeces. “Butyrate is interesting because it’s one of the principal fuel components for the cells that line your gut,” Mohr says. “Increasing their health is obviously important for gastrointestinal health overall.”
A study published the following year looked at differences between the microbiomes of amateur and professional cyclists, using the more sensitive shotgun sequencing technique. The researchers didn’t find systematic differences between amateurs and professionals, but they did find that self-reported exercise duration was associated with levels of the bacterial genus Prevotella. Levels of Prevotella also correlated with certain processes in the gut, including the metabolism of carbohydrates and branched-chain amino acids.
Some press coverage of the study, however, was problematic. The possibility that boosting the level of a specific microbe in a cyclist’s gut could increase performance led to stories trumpeting the imminent arrival of ‘poop doping’. But not only was the sample used in the study too small to draw such strong conclusions, it also wasn’t random. The authors recruited friends, who probably shared many other things in common, such as geographic location, social interactions and diet, which could result in similarities in their microbiomes. “That’s a huge confounding factor,” says Jonathan Eisen, director of the Microbiome Special Research Program at the University of California, Davis, who was one of several researchers who pushed back against the media coverage.
The difficulty of untangling the contribution of diet was highlighted in a 2014 study of professional rugby players. Using 16S rRNA gene sequencing, the authors found greater diversity in gut microbiota in the players than in non-athletes matched for age, gender and BMI. However, the team also noted that there were extreme dietary differences between the two groups. This was especially true of protein intake, which also correlated with microbial diversity. “We couldn’t quite separate exercise from the accompanying dietary changes, to see which was having the greater effect,” says one of the authors, gastroenterologist Fergus Shanahan at University College Cork, Ireland.
In 2018, Shanahan and his colleagues analysed the same samples again, but this time they used shotgun sequencing. The researchers found a clearer separation between athletes and non-athletes in terms of gene function, including differences in carbohydrate metabolism, amino-acid synthesis and the production of SCFAs. The study illustrates the power of shotgun sequencing for assembling the puzzle of microbiome function. “It’s very much a product of technology development,” says Kostic. “Even though bacteria might be taxonomically very diverse, oftentimes their basic functions are quite similar.”
Scheiman’s Boston marathon study was longitudinal from the outset. Samples were collected daily, before and after the marathon, in an effort to study the acute effects of exercise on the microbiome. The researchers used 16S rRNA sequencing, and one genus in particular caught their attention. “The thing that really jumped out was a spike in Veillonella abundance immediately after the marathon,” says Kostic. “We also noticed that Veillonella was generally higher amongst the runners, relative to sedentary controls.” With just 15 runners and 10 control participants, the sample size was limited, but the team also replicated the finding in an independent cohort of elite rowers and runners using shotgun sequencing. The task then was to work out why.
Veillonella is known to metabolize lactate, a by-product of anaerobic respiration that is responsible for the aching sensation in the muscles that can follow intense exercise. When the team analysed samples from the second cohort, it found post-exercise spikes in the expression of genes that encode proteins involved in the metabolic pathway that Veillonella uses to convert lactate into propionate. “This was one of those results where a light bulb goes off, and it just makes sense,” says Kostic. Lactate is a metabolite that would be produced in abundance during a marathon, and that Veillonella can use for energy. And the propionate produced by Veillonella promotes muscle function. The presence of these bacteria might, therefore, create a feedback loop that benefits athlete and bacteria alike. “Someone exercising regularly is creating this metabolic niche for lactate-utilizing bacteria like Veillonella,” says Kostic.
The importance of microbes that produce SCFAs has been hinted at by several studies, but only ever as an association. So, with a strong theory to test, the team conducted some experiments. First, it isolated a Veillonella strain from a runner, inoculated mice with it and then measured how long the rodents could run on a treadmill. Compared with mice inoculated with a bacterium that doesn’t metabolise lactate (Lactobacillus bulgaricus), the Veillonella-supplemented mice ran for 13% longer. It is possible that the control bacterium was having a detrimental effect, rather than Veillonella having a positive one, but a final experiment showed that administering propionate directly to mice had a similar effect on performance, compared with a saline control. “This study stands out” in taking the crucial step from correlation to causation, says Mohr.
]]>
Bad night’s sleep? It’s not uncommon to crave the comfort of a full-fat, sugar-encrusted doughnut. Equally, a large dinner of fried food might leave you tossing and turning in bed and waking drained the next day. But food isn’t necessarily the common denominator here. Increasingly, evidence suggests that the gut microbiome – the bacteria and other micro-organisms in our digestive tract – can influence the quality of our sleep.
Research into the microbiome and its role in health and behaviour has been a hot topic for several years, with research suggesting links between gut bacteria and various diseases and mental health disorders, including cancer and depression. Now scientists believe that there seems to be a relationship with sleep too.
Dr David Gozal, professor of child health, medical physiology and pharmacology at the University of Missouri School of Medicine, is one of those scientists.
“Sleep is very likely to influence the gut microbiome,” he says. “Conversely, it is very likely that changes in the microbiome will influence sleep.”
Read full article here.
]]>How can it be that wealthy countries such as the USA or Western European nations that have good medical infrastructures are among the hardest hit by the virus? That is the question the microbiologist Heenam Stanley Kim, from Korea University’s Laboratory for Human-Microbial Interactions in Seoul, kept asking himself about the COVID-19 pandemic.
Several factors can be put forward to explain the pandemic numbers in western countries, such as lockdown policies and implemented testing strategies, as well as an ageing population. However, Kim brings a whole other explanation to the table focussing on his own expertise: microbiology.
All these countries also have in common the so-called Western diet, high in processed foods and refined sugars, but low in fiber. This diet could potentially be involved in reducing the gut microbiota’s diversity. Loss of diversity has been observed in patients with chronic conditions. So, thought Kim, what if a weak gut microbiota was also linked to more severe COVID-19? And, in reverse, a more diverse diet, rich in fiber, fermented foods and probiotics, could have a protective role?
Kim explores this hypothesis in a review published recently in the journal of the American Society for Microbiology, mBio. He examines emerging evidence that suggests that poor gut health can negatively affect COVID-19 prognosis.
According to Kim, if the virus is able to penetrate the gastrointestinal cells, it can worsen a patient’s outcome, and a weak gut microbiota makes it easier for the virus to enter the organism.
In fact, despite mainly being viewed as a respiratory disease, COVID-19 can also reach other organs such as the kidneys, liver, heart, brain and the gastrointestinal tract.
Read the full article here.
A new study, published in the Journal of the American College of Cardiology, highlights how certain diets can decrease the risk of cardiovascular disease. Researchers have found that following a diverse and healthy diet, rich in plants can decrease the risk of heart disease by reducing levels of a molecule called trimethylamine N-oxide (TMAO). TMAO is produced when the gut microbiota digests nutrients commonly found in red meat and is one of the molecules that forms atheromatous plaques in the blood, leading to an increased risk of cardiac or circulatory system conditions, such as heart attack or stroke.
Find the full article here.
]]>For example, a diet rich in protein and fat will result in more Bacteroidetes being present in your gut microbiome. Studies show that one day only of a vegan diet can alter you’re your microbiome community. However, it quickly reverts back to normal if the diet isn’t sustained. Two parts of our diet that are capable to uniquely modify our microbiome are prebiotics and probiotics. Probiotics are live microorganism that offer health benefits, which are naturally already present in our guts.
You can check our latest article about which food is rich in those. Prebiotics are food components that are used by host microbes. Usually, those high fibers make their way to the intestine without being broken down and fermented in turn there by gut bacteria. Prebiotics are basically food for our gut’s microbes.
If you’re curious about your unique microbiome composition and what food to eat for it to perform best and improve your health get a Nylos kit today.
However, did you know that your gut microbiome also affects your mental health? These tiny beings help you break down food traveling through your intestines, hence producing metabolites influencing all your cells – including those of your nervous system. Simultaneously, immune responses to harmful pathogens produce molecules that can also affect brain physiology. But that’s just scratching the surface. A healthy and diverse microbiome is essential for normal cognitive and emotional processing. Your microbiome communicates with the central nervous system – aka the brain and spinal cord – through nervous, endocrine, and immune signalling mechanisms. We don’t yet have a good understanding of how the gut microbiome and central nervous system influence one another, but it’s been shown that changes in gut flora composition can result in increased intestinal permeability, allowing neuroactive compounds through and activating the inflammatory response. Yet other microbiota can produce compounds that affect gene expression in the nervous system. Research has shown that changes in microbiota can cause depression, change social interactions, protect from stress-induced changes to the immune system, and can cause physiological changes that are even transferable between species!
Our lifestyle has a major effect on the composition of our microbiome. What we eat, our stress levels, and our emotional state determine which organisms can live on in our gut. The human gut microbiota is generally fairly stable, and resists change in community makeup. However, the brain can modulate the composition of our gut community by changing intestinal permeability and secretions, as well as through the release of hormones that affect microbial gene expression. Our gut flora composition can also be perturbed by changes in hormones or diet, antibiotics and stress. Reduction of the normal gut biota population – for instance while taking antibiotics – provides an opportunity for pathogens to colonize the gut epithelium. It has been known for a while that the gastrointestinal system communicates with the brain. The enteric nervous system is a mesh-like set of 500 million neurons governing the gastrointestinal tract. That’s 5 times as many neurons as there are in your spinal cord – no wonder the enteric nervous system is sometimes called the second brain! The enteric nervous system CAN operate autonomously, however, it communicates with the central nervous system via the vagus nerve and prevertebral ganglia.
This biochemical signalling between the gastrointestinal tract and central nervous system is called the gut-brain axis. However, it is only now being realized just how much of an affect the microbiome has on the brain. Hence, this bidirectional interaction between the microbiome and the central nervous system has been termed the microbiome-gut-brain axis. The gut microbiome and central nervous system have bidirectional effects on one another.
More research on this topic will help us get further insights into disorders of both the gut and the central nervous system. This is exciting news, because perhaps neuropsychiatric disorders will one day be treated through gut microbiota! Preclinical studies have identified plainly the powerful influence of gut microbiota on the central nervous system, but there are still issues with reproducibility, so we need continued improvement of experimental approaches.
So, what can you do to maintain the health of your gut flora? Eat a healthy diet tailored to your unique gut flora composition.
Scientists are exploring evidence that major depression may in part be a gut feeling, orchestrated by the microbiome—trillions of microorganisms living in and around our bodies, which influence our health and well-being.
New studies point to the trillions of organisms in the human microbiome as playing an unexpectedly huge role in our well-being
Scientists are exploring evidence that major depression may in part be a gut feeling, orchestrated by the microbiome—trillions of microorganisms living in and around our bodies, which influence our health and well-being.
In a series of studies, researchers are discovering that the microbial menagerie living in our digestive tract may help regulate brain function, including mental health. Recent findings by scientists in the U.S., Europe and China are linking our feelings of stress, anxiety and severe depression to disturbances among hundreds of microbe species living in our gut that some researchers have started calling the psychobiome.
Conversely, other bacteria in the gut appear to produce some of the same substances used by doctors to treat depression and may naturally play a role in maintaining our emotional balance.
By Robert Lee Hotz
]]>